Journal: Clinical Journal of the American Society of Nephrology : CJASN

Article Title: Expression of CD147 and Cyclophilin A in Kidneys of Patients with COVID-19

doi: 10.2215/CJN.09440620

Figure Lengend Snippet: The dysregulated CD147 and cyclophilin A (CypA) in kidney from COVID-19 cases. (A) The basolateral distribution of CD147 (1:6000, 10186-R125; Sino Biologic, Beijing, China) in the patient with glomerulitis without diagnostic morphologic abnormalities. (B) The staining of CD147 in autopsied tissue with acute tubular injury (ATI). (C) The CD147 expression in a deceased donor with ATI. (D and E) In patients with coronavirus disease 2019 (COVID-19), the distribution of CD147 shifts from basolateral to circumferential pattern. (F) The staining of CypA (1:200, sc-134310; Santa Cruz Biotechnology) in a patient without COVID-19 and without ATI. (G) The staining of CypA in autopsied tissue with ATI. (H and I) The staining of CypA in autopsied kidney tissues from patients with COVID-19 (the density of positively stained areas was evaluated by Image-Pro Plus 6.0 software; Media Cybernetics, Rockville, MD). Magnification, ×200 in A–E, G, and H; ×400 in F and I.

Article Snippet: The dysregulated CD147 and cyclophilin A (CypA) in kidney from COVID-19 cases . (A) The basolateral distribution of CD147 (1:6000, 10186-R125; Sino Biologic, Beijing, China) in the patient with glomerulitis without diagnostic morphologic abnormalities. (B) The staining of CD147 in autopsied tissue with acute tubular injury (ATI). (C) The CD147 expression in a deceased donor with ATI. (D and E) In patients with coronavirus disease 2019 (COVID-19), the distribution of CD147 shifts from basolateral to circumferential pattern. (F) The staining of CypA (1:200, sc-134310; Santa Cruz Biotechnology) in a patient without COVID-19 and without ATI. (G) The staining of CypA in autopsied tissue with ATI. (H and I) The staining of CypA in autopsied kidney tissues from patients with COVID-19 (the density of positively stained areas was evaluated by Image-Pro Plus 6.0 software; Media Cybernetics, Rockville, MD).

Techniques: Diagnostic Assay, Staining, Expressing, Software

Journal: Oncology Letters

Article Title: Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

doi: 10.3892/ol.2012.981

Figure Lengend Snippet: Correlation between EMMPRIN/CD147 expression and clinicopathological characteristics.

Article Snippet: The sections were blocked with 5% bovine serum albumin (Zhongshan, Beijing, China) in PBS solution for 20 min and probed with rabbit anti-human EMMPRIN/CD147 antibodies (1:300; Boster) at 4°C for 12 h. After washing, the bound antibodies were detected with biotinylated goat anti-rabbit IgG (1:100) and SABC complex at 30°C for 20 min.

Techniques: Expressing

Journal: Advanced Healthcare Materials

Article Title: Droplet Microarray Based Screening Identifies Proteins for Maintaining Pluripotency of hiPSCs

doi: 10.1002/adhm.202200718

Figure Lengend Snippet: Proteins used in this study with their corresponding structures and Protein Data Bank (PDB) codes

Article Snippet: Proteins (Thy‐1, EphB4, EphA1, E‐cadherin, coxackie and adenovirus receptor (CAR), JAM1, EpCAM, basigin (BSG), and dystroglycan (DAG1)) and rabbit anti‐CD147 antibody were purchased from Sino Biological Europe GmbH (Eschborn, Germany).

Techniques: Marker, Membrane, Protein Binding, Activation Assay, Migration, Stem Cell Culture

Journal: bioRxiv

Article Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein

doi: 10.1101/2020.03.14.988345

Figure Lengend Snippet: (A) The interaction of CD147 and SP detected by SPR assay, KD = 1.85×10 −7 M. (B) The interaction of CD147 and SP detected by Co-IP assay. Anti-CD147 antibody and anti-SARS-CoV-2 Spike antibody were used for antibody immobilization for Co-IP. The mIgG and rIgG were selected as negative control. (C) The interaction of CD147 and SP detected by ELISA. (D) The ability of Meplazumab to compete with SP for CD147 binding performed by competitive inhibition ELISA.

Article Snippet: Anti-CD147 antibody (HAb18, produced by our laboratory) and anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) were used for antibody immobilization for Co-IP.

Techniques: SPR Assay, Co-Immunoprecipitation Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition

Journal: bioRxiv

Article Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein

doi: 10.1101/2020.03.14.988345

Figure Lengend Snippet: (A) The SARS-CoV-2 infected Vero E6 cell with visible SARS-CoV-2 virion (black arrow). ( B and C ) The localization of CD147 (10nm-gold colloid, yellow arrows) and SP (20nm-gold colloid, red arrows) in viral inclusion bodies of SARS-CoV-2 infected Vero E6 cell. Scale bar = 200nm.

Article Snippet: Anti-CD147 antibody (HAb18, produced by our laboratory) and anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) were used for antibody immobilization for Co-IP.

Techniques: Infection